Shortly thereafter, Lois and Clark confer on the brooch and Superman leaves to seek answers.  As he department, his sonic wake again activates the brooch and compels Lana to act, this time with Lois as a witness.  Following her, she sees Lana enlarge a mosquito into a mount and take off after Superman.  Again, she engages him - this time with an army of giant ants - but realizing he is doing something to draw her attention, Superman feigns defeat and drops to the ground.  Once unmoving and silent, Lana again departs.  In the battle, neither notices a ship picking off a straggling giant ant.  The captor is the Ultra-Humanite, in need of a new body and his team of surgeons transplants his brain into the giant ant.  Lana reads an article in the Daily Star and notices in the picture of the Insect Queen, her brooch matches her own.  The Ultra-Humanite now in the ant form rushes in and explains that she is indeed the Insect Queen and he intends to use her powers to defeat Superman once and for all.

Using his new wings in a precise vibrational pattern, the Ultra-Humanite brought Lana under his control and sought the deadliest insects he could find.  Meanwhile, Superman has sought out Lewis Lang in Egypt and learned of a counter-remedy that would break the scarab's spell if it was ever used in appropriately.  Return to Metropolis, he finds the Insect Queen on a rampage, this time under the control of his old adversary.  As they battle across the sky, Superman manages to dislodge Lana's brooch, dropping into Lois' hands.  Before the Ultra-Humanite can switch control to Lois, she deploys the Egyptian remedy and breaks the spell on Lana, who stuns the ant housing the Ultra-Humanite by beating it's head until it falls from the sky.  Superman catch Lana before she falls, her ants shrink to normal size and the headless ant of the Ultra-Humanite's body is seen laying on the ground, it's head bound somehow for it's next host .  Lana not had control of her sense when she used the brooch and could become the Insect Queen at will (Superman Family #214-215).

Some months later, Superman receives news of an odd story, of rain falling up instead of down on the ocean. Investigating, Superman finds insectoid aliens at the root of it.  A cross-country chase results in tornados made of fire and geysers made of ice as the aliens wreak havoc.  Superman is eventually captured but not before noticing certain sounds impeded their movement.  Communicating with Lois, he recruited the Insect Queen to help and her powers quelled their violent impulses until she could command them to return to the homeworld (Superman Family #222).

The Insect Queen was seen during the Crisis on Infinite Earths but it is unclear whether she is from earlier in time or concurrent with the events on Earth-Two in the 1980's,  Her activities after the 1950's remain unrevealed.